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KMID : 0380020090240050477
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 5 p.477 ~ p.482
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Extraction and Isolation of Antioxidant Fraction from Waste of Grape Products for Cosmetic Application
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Kim Eui-Jin
Shin Hyun-Jae
Lee Tae-Ho
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Abstract
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Anthocyanin and origomeric proanthocyanidin (OPC) fractions showing antioxidizing activity are present in grape extracts. Grape extracts are widely used in cosmetic applications as functional ingredients. The aim of our study is to isolate the antioxidant fraction from waste of grape products. The extraction was done using soxhlet apparatus. Next, the extraction was subjected for identification of antioxidants by using HPLC. Antioxidant assay was performed by using 2,2-diphenyl-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD) method. Antioxidizing activity was found to be higher in grape seed extract when compared to grape skin extract. Using the extract, a novel formula with multi lamella emulsion structure has been developed and its safety and stability were confirmed by standard protocols.
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KEYWORD
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Extraction, antioxidant fraction, grape waste, anthocyanin, OPC
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